Basidiosporgenesis by Brown-Rot Basidiomycetes in vitro

Basidiospores of wood-rotting basidiomycetes are a primary source of infection causing wood biodeterioration, especially in wood above ground. Most studies evaluating wood preservatives have used mycelia or basidiospores obtained from wild mushrooms. The objecting of this study was to demonmtrate in virto methods that promote carpogenesis and basidiosporogenesis by the brown-rot fungi Antrodia carbonica, Neolentinus lepideus, and Postia plancenta. After preincubation in the dark at 27°C for 3 to 11 days, basidiospores were easily produced in 1 to 4 months by basidiomata exposed to light at 12°C. Adequate light exposure, aeration, and low temperature treatment after preincubation are essential for fruiting body formation of these brown-rot basidiomycetes. The morphology of the basidiomata differed according to the basidiomycetes and the medium used. These results demonstrate that an enormous quantity of bamidiospores can be easily and continuously produced in 2 to 4 months in vitro.